Drug-DNA Interaction Protocols / edited by Keith R. Fox.

Format:

Book

Contributor:

Fox, Keith R., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 613.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 613

Language:

English

Subjects (All):

Medicine.

Human genetics.

Pharmacology.

Biomedicine.

Pharmacology/Toxicology.

Human Genetics.

Local Subjects:

Biomedicine.

Pharmacology/Toxicology.

Human Genetics.

Physical Description:

1 online resource (IX, 311 pages) : 103 illustrations, 1 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2010.

System Details:

text file PDF

Summary:

The last few years have witnessed the creation of new generations of sequence reading compounds, which have incredible potential for targeting specific DNA sequences. In Drug-DNA Interaction Protocols, Second Edition, expert researchers explore these compounds, revealing how a deeper understanding of the structure of DNA, along with an improved ability to manipulate it, has led to much progress in recent years. Chapters describe a new arsenal of techniques for probing the interactions between drugs and DNA, including various biophysical techniques for quantifying these interactions and for describing them in molecular and atomic detail, and they comprehensively consider both molecular and cellular approaches. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and current, Drug-DNA Interaction Protocols, Second Edition, is a critical volume that includes a wide range of chapters, which together provide methods for assessing the strength and mode of binding, the sequence selectivity, and the effect on biological systems.

Contents:

Quantitative Analysis of Small Molecule-Nucleic Acid Interactions with a Biosensor Surface and Surface Plasmon Resonance Detection

Thermal Melting Studies of Ligand DNA Interactions

Circular and Linear Dichroism of Drug-DNA Systems

Drug Binding to DNA?RNA Hybrid Structures

Quantification of Binding Data Using Capillary Electrophoresis

Determination of Equilibrium Association Constants of Ligand-DNA Complexes by Electrospray Mass Spectrometry

Detection of Adriamycin-DNA Adducts by Accelerator Mass Spectrometry

Molecular Modelling Methods to Quantitate Drug-DNA Interactions

Application of Anomalous Diffraction Methods to the Study of DNA and DNA-Complexes

DNase I Footprinting

Methods to Characterize the Effect of DNA-Modifying Compounds on Nucleosomal DNA

REPSA: Combinatorial Approach for Identifying Preferred Drug-DNA Binding Sequences

In vitro Transcription Assay for Resolution of Drug-DNA Interactions at Defined DNA Sequences

In vitro Footprinting of Promoter Regions Within Supercoiled Plasmid DNA

Topoisomerase I-Mediated DNA Relaxation as a Tool to Study Intercalation of Small Molecules into Supercoiled DNA

A High-Throughput Assay for DNA Topoisomerases and Other Enzymes, Based on DNA Triplex Formation

Measurement of DNA Interstrand Crosslinking in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay

Measurement of DNA Interstrand Crosslinking in Naked DNA Using Gel-Based Methods

An Evaluation Cascade for G-Quadruplex Telomere Targeting Agents in Human Cancer Cells.

Other Format:

Printed edition:

ISBN:

9781603274180

Access Restriction:

Restricted for use by site license.