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Lentivirus Gene Engineering Protocols / edited by Maurizio Federico.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 229.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 229
- Language:
- English
- Subjects (All):
- Life sciences.
- Cytology.
- Life Sciences.
- Cell Biology.
- Local Subjects:
- Life Sciences.
- Cell Biology.
- Physical Description:
- 1 online resource (XII, 316 pages).
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2003.
- System Details:
- text file PDF
- Summary:
- Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.
- Contents:
- Lentivirus Vectors
- From Lentiviruses to Lentivirus Vectors
- The Choice of a Suitable Lentivirus Vector
- Choice and Use of Appropriate Packaging Cell Types
- Small- to Large-Scale Production of Lentivirus Vectors
- Detection and Titration of Lentivirus Vector Preparations
- Detection and Selection of Lentiviral Vector-Transduced Cells
- Detection of Replication-Competent Lentiviral Particles
- Transduction of EX Vivo Cell Cultures Through Lentivirus Vectors
- Lymphocytes
- Monocyte/Macrophages and Dendritic Cells
- Hematopoietic Stem and Progenitor Cells
- Mesenchymal Stem Cells
- Hippocampal Neurons
- Dorsal Root Ganglia Sensory Neurons
- Cardiomyocytes
- Airway Epithelia
- Corneal Cells
- Retinal Tissue
- Advantages and Uses of Non-HIV-1-Based Lentivirus Vectors
- HIV-2 Vectors
- SIV Vectors
- FIV Vectors
- Hybrid Lentivirus Vectors
- Direct In Vivo Transduction by Lentivirus Vector and Detection
- Cells of Respiratory Epithelium
- Cells of the Nervous System.
- Other Format:
- Printed edition:
- ISBN:
- 9781592593934
- Access Restriction:
- Restricted for use by site license.
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