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DNA Replication : Methods and Protocols / edited by Sonya Vengrova, Jacob Dalgaard.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Vengrova, Sonya, editor.
Dalgaard, Jacob, editor.
SpringerLink (Online service)
Series:
Methods in molecular biology 1064-3745 ; 1300.
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 1300
Language:
English
Subjects (All):
Medicine.
Medical genetics.
Biomedicine.
Gene Function.
Local Subjects:
Biomedicine.
Gene Function.
Physical Description:
1 online resource (X, 295 pages) : 36 illustrations, 15 illustrations in color.
Edition:
Second edition 2015.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2015.
System Details:
text file PDF
Summary:
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
Contents:
A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells
Microscopy Techniques to Examine DNA Replication in Fission Yeast
High-Resolution Analysis of Mammalian DNA Replication Units
Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing
Measuring DNA Content by Flow Cytometry in Fission Yeast
Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast
Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast
Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection
Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo
Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae
Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin
Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors
Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism
Single-Molecule Observation Of Prokaryotic DNA Replication
Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast
Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis
Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.
Other Format:
Printed edition:
ISBN:
9781493925964
Access Restriction:
Restricted for use by site license.

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