Signal Transduction Immunohistochemistry : Methods and Protocols / edited by Alexander E. Kalyuzhny.

Format:

Book

Contributor:

Kalyuzhny, Alexander E., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 717.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 717

Language:

English

Subjects (All):

Biochemistry.

Monoclonal antibodies.

Biochemistry, general.

Antibodies.

Local Subjects:

Biochemistry, general.

Antibodies.

Physical Description:

1 online resource (XIII, 302 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.

Contents:

Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies

Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry

An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry

Optimized Protocol to Make Phospho-Specific Antibodies that Work

Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins

An Enhanced Antigen Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues

Imaging Techniques in Signal Transduction IHC

Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining

Flow Cytometric Analysis of Cell Signaling Proteins

CytoSys: A Tool for Extracting Cell Cycle-Related Expression Dynamics from Static Data

Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunolocalization Using Phospho-Selective KOR, p38-MAPK, and KIR 3.1 Antibodies

Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors

Novel Multi-Color Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species

Activation and Differentiation of Mesenchymal Stem Cells

Double In situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo

Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays

Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads.

Other Format:

Printed edition:

ISBN:

9781617790249

Access Restriction:

Restricted for use by site license.