Neural Transplantation Methods / edited by Stephen B. Dunnett, Alan A. Boulton, Glen B. Baker.

Format:

Book

Contributor:

Dunnett, S. B. (Stephen B.), editor.

Boulton, A. A. (Alan A.), editor.

Baker, Glen B., 1947- editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 36.

Springer Protocols (Springer-12345)

Neuromethods, 0893-2336 ; 36

Language:

English

Subjects (All):

Neurosciences.

Local Subjects:

Neurosciences.

Physical Description:

1 online resource (XV, 556 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2000.

System Details:

text file PDF

Summary:

In Neural Transplantation Methods, leading experimental neurobiologists and neurosurgeons provide all the technical and practical information required to perform neural transplantation in experimental and neurological research, including a detailed consideration of the issues involved in designing and customizing such methods for specific research purposes. The authors outline the different sources of donor tissues and other materials for implantation, and discuss the practicalities of embryonic staging and dissection, of the storage of cells by hibernation or cryopreservation, of the expansion of precursor cells, and of the genetic manipulation of cells for in vivo or ex vivo gene transfer. Tissue implantation techniques used for experimental animals of different species include: in oculo and demyelination models, microtransplantation of cell suspensions, encapsulation of transplanted cells, implantation into immature animals-especially primates-and labeling transplanted cells for subsequent detection. Special attention is given to the factors determining transplant survival and integration, detailing the role of the blood-brain barrier, the importance of vascularization for proper graft nutrition, the role of trophic factors, and strategies for immune suppression. Neural Transplantation Methods constitutes the single most comprehensive sourcebook of practical information on the effective use of transplantation techniques in experimental neurobiology. Its powerful and flexible techniques will significantly aid all those now working to develop today's powerful new treatments of brain and spinal cord diseases and injuries.

Contents:

Sources of Cells for Transplantation

Dissecting Embryonic Neural Tissues for Transplantation

Pregraft Tissue Storage Methods for Intracerebral Transplantation

Neural Stem Cells for Transplantation

Immortalized Cells

Cells from Testis for Transplantation in the CNS

Engineered Cells and Ex Vivo Gene Transfer

Intracerebral Gene Transfer Using Viral Vectors

Methods of Implantation

Basic Transplantation Methods in Rodent Brain

Intraocular Grafting

Neural Transplantation in the Developing CNS

Preparation of Cell Suspensions for Transplantation

Microtransplantation of Nigral Dopamine Neurons

Application of Polymer-Encapsulated Cell Therapy for CNS Diseases

Neural Transplantation in Parkinsonian Primates

Transplanting Myelinogenic Cells into the CNS

Labeling and Identifying Grafted Cells

Factors in Graft Survival and Function

Neovascularization and the Blood-Brain Barrier in Neural Transplantation

Trophic Influences on Neural Tissue Transplants

Neuroprotective Strategies in Neural Grafting

Death and Survival in CNS Grafting

Immunology

Immunoprotection of Cell and Tissue Implants in the CNS

Immunobiology of Neural Xenotransplantation.

Other Format:

Printed edition:

ISBN:

9781592596904

Access Restriction:

Restricted for use by site license.