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Advances in Plant Ecophysiology Techniques / edited by Adela M. Sánchez-Moreiras, Manuel J. Reigosa.
- Format:
- Book
- Language:
- English
- Subjects (All):
- Plant ecology.
- Plant physiology.
- Biochemistry.
- Cell physiology.
- Agriculture.
- Plant Ecology.
- Plant Physiology.
- Plant Biochemistry.
- Cell Physiology.
- Local Subjects:
- Plant Ecology.
- Plant Physiology.
- Plant Biochemistry.
- Cell Physiology.
- Agriculture.
- Physical Description:
- 1 online resource (XI, 497 pages) : 110 illustrations, 70 illustrations in color
- Contained In:
- Springer eBooks
- Place of Publication:
- Cham : Springer International Publishing : Imprint: Springer, 2018.
- System Details:
- text file PDF
- Summary:
- This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death, and other parameters; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology. Every chapter is focused on the measurement of a parameter from a very practical point of view, including its use in plant ecophysiology and the meaning of the results that can be obtained.
- Contents:
- Preface
- 1. In vitro and in vivo bioassays
- 2. Root morphology
- 3. Plant photosynthetic pigments: Methods and tricks for correct quantification and identification
- 4. Measuring photosynthesis and respiration with infrared gas analysers
- 5. Chlorophyll fluorescence: a practical approach to study ecophysiology of green plants
- 6. Thermoluminescence: a tool to study ecophysiology of green plants
- 7. Determining plant water relations
- 8. Thermal imaging and infrared sensing in plant ecophysiology
- 9. Photoprotection and photo-oxidative stress markers as useful tools to unravel plant invasion success
- 10. Reactive oxygen species and antioxidant enzymatic systems in plants: role and methods
- 11. Flow cytometric measurement of different physiological parameters
- 12. Flow cytometry: cell cycle
- 13. Mitotic index
- 14. Fluorescent probes and live imaging of plant cells
- 15. Confocal and transmission electron microscopy for plant studies
- 16. Plant programmed cell death (PCD): using cell morphology as a tool to investigate plant PCD
- 17. Visualization of plant microtubules
- 18. Multiprobe in-situ hybridization to whole mount Arabidopsis seedlings
- 19. Proteomics analysis of plant tissues based on two-dimensional gel electrophoresis
- 20. Metabolomics and metabolic profiling - investigation of dynamic plant-environment interactions at the functional level
- 21. SAR / QSAR
- 22. Elucidating the phytotoxic potential of natural compounds
- 23. Exploring plants strategies for allelochemical detoxification
- 24. Chemical characterization of volatile organic compounds (VOCs) through headspace solid phase micro extraction (SPME)
- 25. Carbon radiochemicals (14C) and stable isotopes (13C): crucial tools to study plant-soil interactions in ecosystems
- 26. Stable-isotope techniques to investigate sources of plant water
- 27. Soil microorganisms
- 28. Computational approach to study ecophysiology.
- Other Format:
- Printed edition:
- ISBN:
- 9783319932330
- Access Restriction:
- Restricted for use by site license.
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