Synthetic Metabolic Pathways : Methods and Protocols / edited by Michael Krogh Jensen, Jay D. Keasling.

Format:

Book

Contributor:

Krogh Jensen, Michael, editor.

Keasling, Jay D., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1671.

Methods in Molecular Biology, 1064-3745 ; 1671

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (XII, 354 pages) : 68 illustrations, 64 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Metabolic Pathways: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Parts characterization for tunable protein expression

Enzyme nicotinamide cofactor specificity reversal guided by automated structural analysis and library design

Bacterial genome editing strategy for control of transcription and protein stability

An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization

Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design

Extended Metabolic Space Modeling

Computational Methods to Assess the Production

Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-gene Systems

An adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism

CRISPR-Cas9 toolkit for Actinomycete Genome Editing

Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast using CasEMBLR

A modified Gibson assembly method for cloning large DNA fragments with high GC contents

Coupling Yeast Golden Gate and VEGAS for efficient assembly of the violacein pathway in Saccharomyces cerevisiae

Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites For Phenotyping Of Microorganisms

Selection of Highly Expressed Gene Variants in Escherichia coli using Translationally-coupled Antibiotic Selection Markers

Design, Engineering, and Characterization Of Prokaryotic Ligand-binding Transcriptional Activators as Biosensors in Yeast

A Capture-SELEX Strategy for Multiplexed Selection of RNA Aptamers against Small Molecules

High-throughput Microfluidics for the Screening Of Yeast Libraries

Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution

Two scale 13C Metabolic Flux Analysis for Metabolic Engineering.

Other Format:

Printed edition:

ISBN:

9781493972951

Access Restriction:

Restricted for use by site license.