Peptidomics : Methods and Strategies / edited by Michael Schrader, Lloyd Fricker.

Format:

Book

Contributor:

Schrader, Michael, editor.

Fricker, Lloyd, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1719.

Methods in Molecular Biology, 1064-3745 ; 1719

Language:

English

Subjects (All):

Life sciences.

Proteins.

Life Sciences.

Protein Science.

Local Subjects:

Life Sciences.

Protein Science.

Physical Description:

1 online resource (XVII, 423 pages) : 79 illustrations, 17 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls. Authoritative and practical, Peptidomics:Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics. .

Contents:

Origins, Technological Development and Applications of Peptidomics

Brain Tissue Sample Stabilization and Extraction Strategies for Neuropeptidomics

Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry

Mass Spectrometric Identification of Endogenous Peptides

Bioinformatics for Prohormone and Neuropeptide Discovery

Substrate Capture Assay using Inactive Oligopeptidases to Identify Novel Peptides

Non-targeted Identification of d-amino Acid-containing Peptides through Enzymatic Screening, Chiral Amino Acid Analysis and LC-MS

Quantitative Peptidomics: General Considerations

Quantitative Peptidomics with Isotopic and Isobaric Tags

Quantitative Peptidomics using Reductive Methylation of Amines

Metabolic Labeling to Quantify Drosophila neuropeptides and Peptide Hormones

Data Preprocessing, Visualization and Statistical Analyses of Non-targeted Peptidomics Data from MALDI-MS

Affinity Purification of Neuropeptide Precursors from Mice Lacking Carboxypeptidase E Activity

Mass Spectrometry Based Immunopeptidomics for the Discovery of Cancer Neo-antigens

Milk Peptidomics to Identify Functional Peptides and for Quality Control of Dairy Products

Neuropeptidomic Analysis of Zebrafish Brain

Identification, Quantitation, and Imaging of the Crustacean Peptidome

Identification of Endogenous Neuropeptides in the Nematode C. elegans using Mass Spectrometry

EndoProteoFASP as a Tool to Unveil the Peptidome-protease Profile: Application to Salivary Diagnostics

Methodology for Urine Peptidome Analysis Based on Nano-HPLC coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Identification of Components in Frog Skin Secretions with Therapeutic Potential as Anti-diabetic Agents

High-Accuracy Mass Spectrometry Based Screening Method for the Discovery of Cysteine Containing Peptides in Animal Venoms and Toxins

Analysis of the Snake Venom Peptidome

Identification of Peptides in Spider Venom Using Mass Spectrometry

Single Cell Peptidomics: Approach for Peptide Identification by N-Terminal Peptide Derivatization

Peptidomic Identification of Cysteine-Rich Peptides from Plants

Analysis of Endogenous Peptide Pools of Physcomitrella patens Moss

The Bright Future of Peptidomics. .

Other Format:

Printed edition:

ISBN:

9781493975372

Access Restriction:

Restricted for use by site license.