RNA Detection : Methods and Protocols / edited by Imre Gaspar.

Format:

Book

Contributor:

Gaspar, Imre, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1649.

Methods in Molecular Biology, 1064-3745 ; 1649

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (XIV, 492 pages) : 94 illustrations, 78 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

Contents:

The Secret Life of RNA: Lessons from Emerging Methodologies

Quantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)

Identifying the m6A Methylome by Affinity Purification and Sequencing

PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution

Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo

LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing

Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas

Single mRNA Molecule Detection in Drosophila

Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos

Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction

Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification

In Situ Detection of MicroRNA Expression with RNAscope Probes

Padlock Probes to Detect Single Nucleotide Polymorphisms

Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons

Optimizing Molecular Beacons for Intracellular Analysis of RNA

Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH

In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes

Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers

Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe

RNA Live Imaging in the Model Microorganism Ustilago maydis

Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells

Live Imaging of mRNA Synthesis in Drosophila

Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog

Detection of the First Round of Translation: The TRICK Assay

Imaging Translation Dynamics of Single mRNA Molecules in Live Cells

Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding

Isolation and Characterization of Endogenous RNPs from Brain Tissues

Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein-RNA Interactions

RNA Tagging: Preparation of High-Throughput Sequencing Libraries

RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells.

Other Format:

Printed edition:

ISBN:

9781493972135

Access Restriction:

Restricted for use by site license.