Protein Scaffolds : Design, Synthesis, and Applications / edited by Andrew K. Udit.

Format:

Book

Contributor:

Udit, Andrew K., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1798.

Methods in Molecular Biology, 1064-3745 ; 1798

Language:

English

Subjects (All):

Life sciences.

Proteins.

Life Sciences.

Protein Science.

Local Subjects:

Life Sciences.

Protein Science.

Physical Description:

1 online resource (XI, 330 pages) : 76 illustrations, 52 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, and Applications aims to ensure successful results in the study of this vital field.

Contents:

Nanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus

Encapsulation of Active Enzymes within Bacteriophage P22 virus-like Particles

Encapsulation of Exogenous Proteins in Vault Nanoparticles

Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage

Metal Ion-induced Self-Assembly and Packaging of CCMV Nanocapsules

Modification of CCMV Nanocages for Enzyme Encapsulation

Synthesis of Cross-linked 2-aminoethyl Methacrylate in P22 Viral Capsid via Atom-transfer Radical Polymerization

Synthesis of Metal-organic Frameworks on Tobacco Mosaic Virus Templates

TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings

Synthesis of Triangular Silver and Gold Nanoprisms Using Consensus Sequence Tetratricopeptide Repeat Proteins

Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis

Incorporation of Non-canonical Amino Acids into Proteins by Global Reassignment of Sense Codons

Modification of Protein Scaffolds via Copper-catalyzed Azide-Alkyne Cycloaddition

Antibody Modification of p-aminophenylalanine-containing Proteins

Cross-linked Collagen Gels using Gold Nanoparticles

Genipin Cross-linking Of Elastin and Elastin-based Proteins

Characterization of Self-Assembled Protein Scaffolds from Collagen-mimetic Peptides

Design and Characterization of Fibers and Bionanocomposites Using the Coiled-coil Domain of Cartilage Oligomeric Matrix Protein

Preparing Genes for Repetitive Elastin-like Polypeptides Using Gibson Assembly

Peptide Amphiphile Micelles for Vaccine Delivery

Controlled Assembly of the Filamentous Chaperone Gamma-prefoldin into Defined Nanostructures

Advances in the Application of Designed Ankyrin Repeat Proteins (Darpins) As Research Tools and Protein Therapeutics. .

Other Format:

Printed edition:

ISBN:

9781493978939

Access Restriction:

Restricted for use by site license.