Mitochondrial Bioenergetics : Methods and Protocols / edited by Carlos M. Palmeira, António J. Moreno.

Format:

Book

Contributor:

Palmeira, Carlos M., editor.

Moreno, António J., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1782.

Methods in Molecular Biology, 1064-3745 ; 1782

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (XI, 422 pages) : 107 illustrations, 59 illustrations in color.

Edition:

Second edition 2018.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.

Contents:

Overview of Mitochondrial Bioenergetics

Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells

High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria

High-Throughput Analysis of Mitochondrial Oxygen Consumption

Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices

Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria

Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells

Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production

Measurement of Proton Leak in Isolated Mitochondria

Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy

Mitochondrial Permeability Transition Pore and Calcium Handling

Redox Equivalents and Mitochondrial Bioenergetics

Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis

Computational Modeling of Mitochondrial Function from a Systems Biology Perspective

Monitoring the Mitochondrial Dynamics in Mammalian Cells

Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria

Plate-Based Measurement of Respiration by Isolated Mitochondria

Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells

The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue

Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells

Imaging of Mitochondrial pH Using SNARF-1

Relation between Mitochondrial Membrane Potential and ROS Formation

Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks

Measurement of Mitochondrial ROS Formation.

Other Format:

Printed edition:

ISBN:

9781493978311

Access Restriction:

Restricted for use by site license.