Innate Antiviral Immunity : Methods and Protocols / edited by Karen Mossman.

Format:

Book

Contributor:

Mossman, Karen, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1656.

Methods in Molecular Biology, 1064-3745 ; 1656

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (XI, 275 pages) : 41 illustrations, 30 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.

Contents:

The Application of Humanized Mouse Models for the Study of Human Exclusive Viruses

Zebrafish as a Model for the Study of Host-Virus Interactions

Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Non-Radioactive Oligo Probes

Extraction and qPCR-Based Detection of miRNAs from Cultured PBMCs of Bubaline Origin

Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods

RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity

Methods to Visualize MAVS Subcellular Localization

Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture

cGAMP Quantification in Virus-Infected Human Monocyte-Derived Cells by HPLC-Coupled Tandem Mass Spectrometry

Methods of Assessing STING Activation and Trafficking

Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells

High Throughput Screening for Identification of Novel Innate Immune Activators

Chromosome Conformation Capture for Research on Innate Antiviral Immunity

Discovery of Variants Underlying Host Susceptibility to Virus Infection Using Whole-Exome Sequencing

Isolation, Purification, and Culture of Primary Murine Sensory Neurons

Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs

Epidemiological Methods.

Other Format:

Printed edition:

ISBN:

9781493972371

Access Restriction:

Restricted for use by site license.