Reporter Gene Imaging : Methods and Protocols / edited by Purnima Dubey.

Format:

Book

Contributor:

Dubey, Purnima, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1790.

Methods in Molecular Biology, 1064-3745 ; 1790

Language:

English

Subjects (All):

Medicine.

Radiology.

Medicine & Public Health.

Imaging / Radiology.

Local Subjects:

Medicine & Public Health.

Imaging / Radiology.

Physical Description:

1 online resource (XI, 221 pages) : 53 illustrations, 42 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Dual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function

Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging

Optical Bioluminescence Protocol for Imaging Mice

Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase

Reporter Based BRET Sensors for Measuring Biological Functions In Vivo

Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains

In vivo Bacterial Imaging using Bioluminescence

Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging

Hypoxia-induced Reporter Genes with Different Half-lives

Visualization of Immune System Reconstitution by Bioluminescent Imaging

Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo

Ex vivo radiolabeling and in vivo PET imaging of T cells expressing nuclear reporter genes

Imaging T Cell Dynamics and Function by PET using Human Nuclear Reporter Genes

Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [18F]C-SNAT

Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes

Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models.

Other Format:

Printed edition:

ISBN:

9781493978601

Access Restriction:

Restricted for use by site license.