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Site-Specific Recombinases : Methods and Protocols / edited by Nikolai Eroshenko.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 1642.
- Methods in Molecular Biology, 1064-3745 ; 1642
- Language:
- English
- Subjects (All):
- Medicine.
- Human genetics.
- Biomedicine.
- Human Genetics.
- Local Subjects:
- Biomedicine.
- Human Genetics.
- Physical Description:
- 1 online resource (XI, 342 pages) : 50 illustrations, 25 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana Press, 2017.
- System Details:
- text file PDF
- Summary:
- This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.
- Contents:
- Generating Genetically Modified Mice: A Decision Guide
- Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes
- Building Cre Knockin Rat Lines using CRISPR/Cas9
- Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
- Use of the DICE Dual Integrase Cassette Exchange System
- Ligand-controlled Site-specific Recombination in Zebrafish
- Injection-based Delivery of Cell Permeable Peptide-tagged Cre
- Viral Delivery of GFP-dependent Recombinases to the Mouse Brain
- Recombinase-mediated Cassette Exchange using Adenoviral Vectors
- Marker Removal in Transgenic Plants using Cre recombinase Delivered with Potato Virus X
- Nanoparticle-mediated Recombinase Delivery into Maize
- Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
- FLPing Genes On and Off in Drosophila
- Imaging Neural Architecture in Brainbow Samples
- Purification and in vitro characterization.
- Other Format:
- Printed edition:
- ISBN:
- 9781493971695
- Access Restriction:
- Restricted for use by site license.
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