MicroRNA Technologies / edited by Min Jeong Kye.

Format:

Book

Contributor:

Kye, Min Jeong, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 128.

Neuromethods, 0893-2336 ; 128

Language:

English

Subjects (All):

Medicine.

Neurosciences.

Biomedicine.

Local Subjects:

Biomedicine.

Neurosciences.

Physical Description:

1 online resource (XII, 216 pages) : 20 illustrations, 10 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals. Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.

Contents:

Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord

Protocol for High Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons

Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-Radioactive Fluorescence Method

Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Protocol

MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation

Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation

Functional Analysis of Cortical Neuron Migration Using miRNA Silencing

Continuous Delivery of Oligonucleotides into the Brain

Isolating and Screening Subcellular miRNAs in Neuron

Experimental Methods for Functional Studies in MicroRNAs in Animal Models of Psychiatric Disorders

Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs

In Ovo Electroporation of miRNA Plasmids to Silence genes in a Temporally and Spatially Controlled Manner

Three Dimensional Cell Culture of Human Neural Stem Cells using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses

Measuring miRNA Mediated Translational Regulation with Live Cell Imaging.

Other Format:

Printed edition:

ISBN:

9781493971756

Access Restriction:

Restricted for use by site license.