Planarian Regeneration : Methods and Protocols / edited by Jochen C. Rink.

Format:

Book

Contributor:

Rink, Jochen C., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1774.

Methods in Molecular Biology, 1064-3745 ; 1774

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (XI, 573 pages) : 92 illustrations, 56 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.

Contents:

Planarian Diversity and Phylogeny

Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale

The Ecology of Freshwater Planarians

Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology

Culturing Planarians in the Laboratory

Total RNA Isolation from Planarian Tissues

Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians

Planarian High Molecular Weight DNA Isolation by Spooling

Basic Histological Techniques for Planarians

Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians

Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections

Whole-Mount In Situ Hybridization of Planarians

RNA In Situ Hybridization on Planarian Paraffin Sections

General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods

Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians

Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL

Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria

RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA

A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells

Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation

Tissue Transplantations in Planarians

Live Imaging Planaria

Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques

Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC

Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.

Other Format:

Printed edition:

ISBN:

9781493978021

Access Restriction:

Restricted for use by site license.