Urothelial Carcinoma : Methods and Protocols / edited by Wolfgang A Schulz, Michèle J. Hoffmann, Günter Niegisch.

Format:

Book

Contributor:

Schulz, Wolfgang A., editor.

Hoffmann, Michèle J., editor.

Niegisch, Günter, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1655.

Methods in Molecular Biology, 1064-3745 ; 1655

Language:

English

Subjects (All):

Medicine.

Cancer--Research.

Cancer.

Biomedicine.

Cancer Research.

Local Subjects:

Biomedicine.

Cancer Research.

Physical Description:

1 online resource (XIV, 362 pages) : 73 illustrations, 49 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume details standard techniques for the characterization of urothelial carcinoma as well as methods to investigate mechanisms of carcinogenesis. Chapters guide readers on cellular and animal models for urothelial carcinoma and related diseases, molecular analyses from body fluids, and new approaches to therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Urothelial Carcinoma: Methods and Protocols hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice.

Contents:

Analysis of Chromosomal Alterations in Urothelial Carcinoma

Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma

A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer

Immunohistochemical Analysis of UC Tissues for Proliferation and Differentiation Markers

Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel

Defining the Pathways of Urogenital Schistosomiasis-associated Urothelial Carcinogenesis through Transgenic and Bladder Wall Egg Injection Models

Algorithm for the Automated Evaluation of NAT2 Genotypes

Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma

Oxidative Stress in Urothelial Carcinogenesis: Measurements of Protein Carbonylation and Intracellular Production of Reactive Oxygen Species

Urothelial Carcinoma Stem Cells - current Concepts, Controversies, and Methods

In Vitro Differentiation and Propagation of Urothelium from Pluripotent Stem Cell Lines

Spheroid Cultures of Primary Urothelial Cancer Cells: Cancer Tissue-originated Spheroid (CTOS) Method

The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model

Patient-derived Bladder Cancer Xenografts

Orthotopic Mouse Models of Urothelial Cancer

Quantification of microRNAs in Urine-derived Specimens

Quantitative RNA Analysis from Urine using Real Time PCR

DNA Methylation Analysis from Body Fluids

Urinary Protein Markers for the Detection and Prognostication of Urothelial Carcinoma

Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin

Epigenetic Treatment Options in Urothelial Carcinoma

Evaluation of Protein Levels of the Receptor Tyrosine Kinase ErbB3 in Serum

Targeting the PI3K/AKT/mTOR Pathway in Bladder Cancer

Visualization and Quantitative Measurement of Frug-induced Platinum Sdducts in the Nuclear DNA of Individual Cells by an Immuno-cytological Sssay.

Other Format:

Printed edition:

ISBN:

9781493972340

Access Restriction:

Restricted for use by site license.