Handbook of ELISPOT : Methods and Protocols / edited by Alexander E. Kalyuzhny.

Format:

Book

Contributor:

Kalyuzhny, Alexander E., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1808.

Methods in Molecular Biology, 1064-3745 ; 1808

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (X, 251 pages) : 74 illustrations, 68 illustrations in color.

Edition:

Third edition 2018.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot's sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.

Contents:

Challenges in Developing Protein Secretion Assays at a Single-Cell Level

To Count of Not to Count: ELISPOT Evaluation Revisited

Essential Controls for ELISpot Assay

Automatic Search of Spots and Color Classification in ELISPOT Assay

Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages

Detection of Cross-Reactive B Cells Using the FluoroSpot Assay

Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations

Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays

Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm

Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite

B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells

Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFNg ELISPOT

ELISPOT-Based "Multi-Color FluoroSpot" to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections

Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses

Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses

Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection

Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins

Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines

Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay.

Other Format:

Printed edition:

ISBN:

9781493985678

Access Restriction:

Restricted for use by site license.