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Mouse Oocyte Development : Methods and Protocols / edited by Marie-Hélène Verlhac, Marie-Emilie Terret.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 1818.
- Methods in Molecular Biology, 1064-3745 ; 1818
- Language:
- English
- Subjects (All):
- Life sciences.
- Cytology.
- Life Sciences.
- Cell Biology.
- Local Subjects:
- Life Sciences.
- Cell Biology.
- Physical Description:
- 1 online resource (XI, 174 pages) : 43 illustrations, 30 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana Press, 2018.
- System Details:
- text file PDF
- Summary:
- This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
- Contents:
- Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse
- In vitro Culture of Mouse Oocytes for Meiotic Maturation
- In vitro Maturation and Culture of Human Oocytes
- Pronuclear Transfer in Human Oocytes
- In vitro Fertilization of Mouse Oocyte
- Profiling Maternal mRNA Translation during Oocyte Development
- Transcriptome Profiling of Single Mouse Oocytes
- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation
- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation
- Triple-colour Live Imaging of Mouse Oocytes
- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes
- Manipulating Cohesin Levels in Live Mouse Oocytes
- Optogenetic Manipulation of Mouse Oocytes
- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin
- Photoactivation of Actin in Mouse Oocyte
- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes
- Micropipette Aspiration of Oocytes to Assess Cortical Tension.
- Other Format:
- Printed edition:
- ISBN:
- 9781493986033
- Access Restriction:
- Restricted for use by site license.
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