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Recombinant Glycoprotein Production : Methods and Protocols / edited by Virgínia Picanço-Castro, Kamilla Swiech.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Picanco-Castro, Virginia, editor.
Swiech, Kamilla, editor.
SpringerLink (Online service)
Series:
Methods in molecular biology 1064-3745 ; 1674.
Methods in Molecular Biology, 1064-3745 ; 1674
Language:
English
Subjects (All):
Life sciences.
Proteins.
Life Sciences.
Protein Science.
Local Subjects:
Life Sciences.
Protein Science.
Physical Description:
1 online resource (XI, 285 pages) : 51 illustrations, 37 illustrations in color.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2018.
System Details:
text file PDF
Summary:
This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents:
Platforms for Recombinant Therapeutic Glycoprotein Production
Uncovering Innovation Features and Emerging Technologies in Molecular Biology through Patent Analysis
Production of Full-length Antibody by Pichia Pastoris
Human cells as Platform to Produce Gamma-Carboxylated Proteins
Production of Recombinant Factor VIII in Human Cell Lines
Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production
Production of Recombinant Rabies Virus Glycoprotein by Insect Cells in a Single Use Fixed-bed Bioreactor
Cell-Free Production of Protein Biologics within 24 hours
Demonstration-Scale High-Cell-Density Fermentation of Pichia Pastoris
Large Scale Transient Transfection of Suspension Mammalian Cells for VLP Production
Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Cultures
Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production
Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins
Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatography
Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells
Purification Method for Recombinant hG-CSF by Affinity Chromatography
Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification
Purification and Auto Activation Method for Recombinant Coagulation Factor VII
Preparation of Immunoliposomes by Direct Coupling of Antibodies Based on a Thioether Bond
Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies
Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins
Quantification of Coagulation Factor VIII by Selective Reaction Monitoring.
Other Format:
Printed edition:
ISBN:
9781493973125
Access Restriction:
Restricted for use by site license.

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