Functional Genomics : Methods and Protocols / edited by Michael Kaufmann, Claudia Klinger, Andreas Savelsbergh.

Format:

Book

Contributor:

Kaufmann, Michael, editor.

Klinger, Claudia, editor.

Savelsbergh, Andreas, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1654.

Methods in Molecular Biology, 1064-3745 ; 1654

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (XV, 392 pages) : 74 illustrations, 51 illustrations in color.

Edition:

Third edition 2017.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

Reflecting developments in genome editing, this third edition volume fully updates a collection of key techniques for the study of functional genomics. The book is broken up into sections on bioinformatics, DNA, RNA, and protein analysis, as well as a closing section entitled "From Genotype to Phenotype." Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and authoritative, Functional Genomics: Methods and Protocols, Third Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.

Contents:

Predicting RNA Structure with Vfold

RNA Function Prediction

Computational Prediction of Novel miRNAs from Genome-Wide Data

Protein Structure Modeling with MODELLER

Protein Function Prediction

Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C

Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila

DNA Methylation Profiling Using Long-Read Single-Molecule Real-Time Bisulfite Sequencing (SMRT-BS)

Copy Number Variation Analysis by Droplet Digital PCR

MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein-Nucleic Acid Interactions in Solution

Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging

Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy

MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation

Using Firefly® Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification

Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling

Optimized Whole Transcriptome Profiling of Motor Axons

2D-DIGE in Proteomics

STAGE-Diging in Proteomics

Protein Arrays I: Antibody Arrays

Protein Arrays II: Antigen Arrays

Protein Arrays III: Reverse-Phase Protein Arrays

Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry

Virus-Induced Gene Silencing (VIGS) and Foreign Gene Expression in Pisum sativum L. Using the 'One-Step' Bean pod mottle virus (BPMV) Viral Vector

Re-Expressing Epigenetically Silenced Genes by Inducing DNA Demethylation through Targeting of Ten-Eleven Translocation 2 to Any Given Genomic Locus

Knockdown of Rice MicroRNA166 by Short Tandem Target Mimic (STTM)

RNAi-Mediated Knockdown of Protein Expression

Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells

Production, Purification, and Titration of First-Generation Adenovirus Vectors.

Other Format:

Printed edition:

ISBN:

9781493972319

Access Restriction:

Restricted for use by site license.