Characterization of Nanoparticles Intended for Drug Delivery / edited by Scott E. McNeil.

Format:

Book

Contributor:

McNeil, Scott E., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1682.

Methods in Molecular Biology, 1064-3745 ; 1682

Language:

English

Subjects (All):

Medicine.

Pharmacology.

Biomedicine.

Pharmacology/Toxicology.

Local Subjects:

Biomedicine.

Pharmacology/Toxicology.

Physical Description:

1 online resource (XII, 256 pages) : 72 illustrations, 40 illustrations in color.

Edition:

Second edition 2018.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents. The chapters in this book are divided into 5 parts and cover topics such as: advances and obstacles in nanomedicine research; methods to test sterility and endotoxin, physicochemical features, immunological effects, drug release, and in vivo efficacy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Characterization of Nanoparticles Intended for Drug Delivery, Second Edition is a valuable tool for researchers and pharmaceutical and biotechnology developers who are evaluating the clinical potential of nanomedicines in preclinical studies.>.

Contents:

Evaluating Nanomedicines: Obstacles and Advancements

Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test

Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations

Elemental Analysis in Biological Matrices Using ICP-MS

PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection

Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis

Immunoelectron Microscopy for Visualization of Nanoparticles

Imaging of Liposomes by Transmission Electron Microscopy

Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties

In Vitro Assessment of Nanoparticle Effects on Blood Coagulation

In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells

Assessing NLRP3 Inflammasome Activation by Nanoparticles

Analysis of Complement Activation by Nanoparticles

Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo

Analysis of Pro-Inflammatory Cytokine and Type II Interferon Induction by Nanoparticles

Analysis of Nanoparticle Adjuvant Properties In Vivo

In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed Type Hypersensitivity Reactions

Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct

Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer

Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition.

Other Format:

Printed edition:

ISBN:

9781493973521

Access Restriction:

Restricted for use by site license.