Quorum Sensing : Methods and Protocols / edited by Livia Leoni, Giordano Rampioni.

Format:

Book

Contributor:

Leoni, Livia, editor.

Rampioni, Giordano, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1673.

Methods in Molecular Biology, 1064-3745 ; 1673

Language:

English

Subjects (All):

Life sciences.

Microbiology.

Life Sciences.

Local Subjects:

Life Sciences.

Microbiology.

Physical Description:

1 online resource (XVI, 375 pages) : 94 illustrations, 49 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and quantification of quorum sensing signal molecules, methods for the studying of quorum sensing at the molecular, physiological, and population level, as well as identification and characterization of anti-quorum sensing agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Quorum Sensing: Methods and Protocols serves as a comprehensive guide to the most important methodology currently available in the field.

Contents:

Use of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion

Detection of 2-Alkyl-4-Quinolones Using Biosensors

"Hot Stuff": The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals

Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)

Detection of the Bacterial Quorum Sensing Signaling Molecules N-Acyl Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)

Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds

Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus

Ultra Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals

Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode

Detection and Quantification of Butyrolactones from Streptomyces

Fluorescence Quenching Studies of γ-Butyrolactone Binding Protein (CprB) from Streptomyces coelicolor A3(2)

Methods to Study Solo/Orphan Quorum Sensing Receptors

Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases

Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq

Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics

Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells

Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies

Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta

Differential Equations Models to Study Quorum Sensing

Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro

A Co-Culture-Based Approach for Screening Campaigns Aimed at Identifying Novel Pseudomonas aeruginosa Quorum Sensing Inhibitors

A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin

Directed Evolution of Quorum Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from Geobacillus kaustophilus

Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones

Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi through Structure-Based Virtual Screening

Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography.

Other Format:

Printed edition:

ISBN:

9781493973095

Access Restriction:

Restricted for use by site license.