Enzyme kinetics : catalysis & control : a reference of theory and best-practice methods / Daniel L. Purich.

Format:

Book

Author/Creator:

Purich, Daniel L.

Language:

English

Subjects (All):

Enzyme kinetics.

Enzymes.

Biocatalysis.

Kinetics.

Medical Subjects:

Enzymes.

Biocatalysis.

Kinetics.

Physical Description:

xxi, 892 pages : illustrations

Edition:

First edition.

Other Title:

Enzyme kinetics catalysis and control

Place of Publication:

Amsterdam : Elsevier/Academic Press, 2010.

Summary:

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, this book develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes.

Contents:

Introduction to enzyme science

Active sites and their chemical properties

Fundamentals of chemical kinetics

Practical aspects of measuring initial rates and reaction parameters

Initial-rate kinetics of one-substrate enzyme-catalyzed reactions

Initial-rate kinetics of multi-substrate enzyme-catalyzed reactions

Factors influencing enzyme activity

Kinetic behavior of enzyme inhibitors

Isotopic probes of biological catalysis

Probing fast enzyme processes

Regulatory behavior of enzymes

Single-molecular enzyme kinetics

Mechanoenzymes: catalysis, force generation and kinetics.

Notes:

Includes bibliographical references and index.

ISBN:

9780123809247

012380924X

OCLC:

502035554