Methods in protein biochemistry / edited by Harald Tschesche.

Format:

Book

Contributor:

Tschesche, Harald.

Language:

English

Subjects (All):

Proteins--Analysis.

Proteins.

Proteomics--Methodology.

Proteomics.

Proteins--chemistry.

Proteins--analysis.

Proteins--isolation & purification.

Proteomics--methods.

Methodology.

Medical Subjects:

Proteins--chemistry.

Proteins--analysis.

Proteins--isolation & purification.

Proteomics--methods.

Physical Description:

xxiii, 352 pages : illustrations ; 25 cm

Place of Publication:

Berlin : De Gruyter, [2012]

Summary:

Biochemists, biophysicists, and related scientists describe the laboratory methods they use to explore the biochemistry of proteins. The goal is not to cover every technique being used, but to sample a few that have proven effective. The topics include three-phase partitioning, analyzing protein complexes using chemical cross-linking and mass spectrometry, wide-angle X-ray solution scattering, target-oriented peptide arrays in a palliative approach to cystic fibrosis, and the protein epitope mimetic approach to protein-protein interaction inhibitors. Annotation ©2012 Book News, Inc., Portland, OR (booknews.com)

Contents:

Three-phase partitioning

Folding and degradation functions of molecular chaperones

Membrane protein folding in detergents

Glycoprotein folding quality control in the endoplasmic reticulum

Protein import into the intermembrane space of mitochondria

On-membrane identification of gel-resolved proteins by MALDI-MS

Analysis of protein complexes using chemical cross-linking and mass spectrometry

Single-crystal spectroscopy correlated with X-ray crystallography provides complementary perspectives on macromolecular function

Wide-angle X-ray solution scattering (WAXS)

Where purity matters : recombinant versus synthetic peptides in beta amyloid formation

Chemical modification of proteins in living cells

Proteomics of human bronchoalveolar lavage fluid : discovery of biomarkers of chronic obstructive pulmonary disease with difference gel electrophoresis (DIGE) and mass spectrometry

Proteomic of Duchenne muscular dystrophy

Target-oriented peptide arrays in a palliative approach to cystic fibrosis

Probing protein dynamics in vivo using backbone cyclization : bacterial acyl carrier protein as a case study

The protein epitope mimetic approach to protein-protein interaction inhibitors

The structural biology of alpha-1 antitrypsin deficiency and the serpinopathies.

Notes:

Includes bibliographical references and index.

ISBN:

9783110252330

3110252333

9783110252361

3110252368

OCLC:

741340082