Human pluripotent stem cells : a practical guide / Uma Lakshmipathy, Chad C. MacArthur, Mahalakshmi Sridharan, Rene H. Quintanilla.

Format:

Book

Author/Creator:

Lakshmipathy, Uma, author.

MacArthur, Chad C., author.

Sridharan, Mahalakshmi, author.

Quintanilla, Rene H., author.

Contributor:

Wiley InterScience (Online service)

Language:

English

Subjects (All):

Multipotent stem cells.

Stem cells.

Pluripotent Stem Cells.

Medical Subjects:

Pluripotent Stem Cells.

Physical Description:

1 online resource (vi, 165 pages) : illustrations

Place of Publication:

Hoboken, NJ : John Wiley & Sons, 2018.

System Details:

text file

Summary:

Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. Includes helpful tips and tricks that are normally omitted from regular research papers, Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations, Presents specific sections (i.e., reprogramming, differentiation) in a concise and easily digestible manner, Written by experts with extensive experience in stem cell technologies Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs. Book jacket.

Contents:

1 Introduction 1

1.1 Biosafety 1

1.2 Biosafety Levels 2

1.3 Aseptic Technique 2

1.4 Storage 6

1.5 Contamination 7

1.6 Pluripotent Stem Cells 8

1.7 Procedures 9

References 16

2 Pluripotent Stem Cell Culture 17

2.1 Introduction 17

2.2 Materials 18

2.3 Solutions 19

2.4 Methods 21

References 47

3 Reprogramming 49

3.1 Introduction 49

3.2 Materials 49

3.3 Solutions 54

3.4 Methods 57

References 97

4 Characterization 99

4.1 Introduction 99

4.2 Materials 101

4.3 Solutions 103

4.4 Methods 104

References 128

5 Differentiation 131

5.1 Introduction 131

5.2 Materials 131

5.3 Solutions 133

5.4 Methods 135

References 151.

Notes:

Includes bibliographical references and index.

Electronic reproduction. Hoboken, N.J. Available via World Wide Web.

Description based on print version record.

Other Format:

Online version: Lakshmipathy, Uma. Human pluripotent stem cells.

ISBN:

9781119394372

1119394376

Publisher Number:

99975607115

Access Restriction:

Restricted for use by site license.