Basic science methods for clinical researchers / edited by Morteza Jalali, Francesca Y.L. Saldanha, Mehdi Jalali ; foreword by Dame Sally Davies.

Format:

Book

Author/Creator:

Davies, S. C. (Sally Claire), 1949- author of foreword.

Contributor:

Jalali, Morteza, editor.

Saldanha, Francesca Yvonne Louise, editor.

Jalali, Mehdi, editor.

Language:

English

Subjects (All):

Research.

Medical research personnel.

Research Design.

Science.

Medical Subjects:

Research Design.

Science.

Genre:

Electronic books.

Physical Description:

1 online resource : illustrations

Place of Publication:

London : Academic Press, 2017.

System Details:

text file

Contents:

Front Cover; Basic Science Methods for Clinical Researchers; Copyright Page; Dedication; Contents; List of Contributors; Foreword; Preface; About This Book; 1 The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR; Introduction; In Principle; Analysis of the PCR Product; Phases of PCR; Variations of PCR Method: Quantitative PCR; Variations of PCR Method: Reverse Transcription PCR; In Practice; Extraction of Total RNA; Synthesis of cDNA by Reverse Transcription; Real-Time PCR Amplification; Data Analysis: Generating a Standard Curve; Relative Quantification (Pfaffl Method); Applications of PCR.

ScenarioKey Limitations; Troubleshooting; Little or No PCR Product; Efficiency of Reaction Is Too Low; Efficiency of Reaction Is Too High; Multiple Bands On Gel, Or Multiple Peaks On Melting Curve; No RT Control Produces a Band; Negative PCR Control Produces a Band; Conclusion; References; Suggested Further Reading; Glossary; List of Acronyms and Abbreviations; 2 Methods of Cloning; Introduction; In Principle; Selection of the Host Organism; Selection of the Cloning Vector; Preparation of the Vector; Preparation of the Insert; Generation of the Recombinant DNA.

Introduction of the Recombinant DNA into the Host OrganismSelection of the Clones of Organisms Containing the Vectors; Screening for Clones with the Desired Recombinant DNA€Molecules; Expansion and Isolation of the Recombinant DNA; In Practice; Selection of the Host Organism; Selection of Cloning Vector; Preparation of the Vector; Preparation of the Insert; Generation of the Recombinant DNA; Introduction of the Recombinant DNA into the Host Organism; Selection of the Clones of Organisms Containing the Vectors; Screening for Clones with the Desired Recombinant DNA Molecules.

Expansion and Isolation of the Recombinant DNAApplications; Study of Gene Function; Study of Genomic Regulatory Regions; Translational Applications; Scenario; Key Limitations; Troubleshooting; Conclusions; Acknowledgments; References; Glossary; 3 Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos; Introduction; In Principle; In Practice-Xenopus Whole-Mount In Situ Protocol-Baskets; Applications; Scenario; Key Limitations; Conclusion; References; List of Acronyms and Abbreviations; 4 Microarrays: An Introduction and Guide to Their Use; Introduction.

In PrincipleIn Practice; Experimental Design Planning; Sample Preparation; Hybridization, Washes, and Scanning; Data Analysis; Result Validation; Applications; Supervised versus Unsupervised Analyses; Analysis of Genomic DNA Features and DNA-Protein Interactions; Scenario; Key Limitations; Sensitivity, Specificity, and Dependence on Known Sequences; Limitations Shared with RNA-Seq; Practical Advantages That Remain; Troubleshooting; Conclusion; References; Suggested Further Reading; Glossary; List of Acronyms and Abbreviations; 5 Analysis of Human Genetic Variations Using DNA Sequencing.

Notes:

Includes bibliographical references and index.

Online resource; title from PDF title page (EBSCO, viewed April 13, 2017).

Other Format:

Print version: Basic science methods for clinical researchers.

ISBN:

9780128030783

012803078X

OCLC:

981463360

Access Restriction:

Restricted for use by site license.