Receptor and Ion Channel Detection in the Brain : Methods and Protocols / edited by Rafael Luján, Francisco Ciruela.

Format:

Book

Author/Creator:

SpringerLink (Online service)

Contributor:

Luján, Rafael, editor.

Ciruela, Francisco, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 110.

Neuromethods, 0893-2336 ; 110

Language:

English

Subjects (All):

Medicine.

Neurosciences.

Neurobiology.

Biology--Technique.

Biology.

Physical Description:

1 online resource (XVIII, 475 pages) : 84 illustrations, 57 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

Contents:

Preface

Production of high-quality antibodies for the study of receptors and ion channels in brain

Co-immunoprecipitation from brain

Sub-synaptic membrane fractionation

Investigation of neurotransmitter receptors in brain slices using cell surface biotinylation

Single nanoparticle tracking of surface ion channels and receptors in brain cells

Radioligand binding detection of receptors in brain membranes

Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain

Fluorescent ligands and TR-FRET to study receptor-receptor interactions in the brain

In situ Proximity Ligation Assay to study and understand the distribution and balance of GPCR homo- and heteroreceptor complexes in the brain

Fluorescent in situ hybridization for sensitive and specific labeling

Autoradiographic visualization of G protein-coupled receptors in brain

Analysis of the expression profile and regional distribution of neurotransmitter receptors and ion channels in the central nervous system using histoblots

Immunohistochemistry for ion channels and their interacting molecules: Tips for improving antibody accessibility

Localization of GFP-tagged proteins at the electron microscope

Pre-embedding methods in the localization of receptors and ion channels

Post-embedding immunohistochemistry in the localization of receptors and ion channels

High-Resolution localization of membrane proteins by SDS-digested freeze-fracture replica labelling (SDS-FRL)

Application of virus vectors for anterograde tract-tracing and single-neuron labeling studies

Analysis of synaptic connections using viral vectors at the electron microscopic level

Morphological and neurochemical characterization of electrophysiologically identified cells

Using electrophysiology to study synaptic and extrasynaptic ionotropic receptors in hippocampal neurons

Biophysical methods to analyze direct G-protein regulation of neuronal voltage-gated calcium channels

Electrophysiological recordings in behaving animals

Voltammetry in behaving animals

In vivo brain microdialysis of monoamines

Calcium transients in single dendrites and spines of pyramidal neurons in vitro

Dynamic recording of membrane potential from hippocampal neurons by using a FRET-based voltage biosensor

Determination of GPCR-mediated cAMP accumulation in rat striatal synaptosomes

GPCR-mediated MAPK/ERK cascade activation in brain slices

Index.

Other Format:

Printed edition:

ISBN:

9781493930647

Access Restriction:

Restricted for use by site license.