Campylobacter jejuni : Methods and Protocols / edited by James Butcher, Alain Stintzi.

Format:

Book

Contributor:

Butcher, James (Post-doctoral Fellow), editor.

Stintzi, Alain, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1512.

Methods in Molecular Biology, 1064-3745 ; 1512

Language:

English

Subjects (All):

Life sciences.

Microbiology.

Physical Description:

1 online resource (XI, 274 pages) : 45 illustrations, 27 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

Campylobacter jejuni (C. jejuni) is often regarded as the one of the most common causes of bacterial gastroenteritis worldwide. The goal of this volume is to highlight key protocols for working with C. jejuni. In particular, chapters aim to highlight recent developments with regards to in vivo models for C. jejuni pathogenesis, different approaches to isolate Campylobacter, and a systems biology approach for studying the effect of all potential Campylobacter gene mutants.Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, <Campylobacter jejuni: Methods and Protocols encourages existing Campylobacter researchers to employ novel methods to further their own research and also encourages new researchers to include Campylobacter in their future research initiatives.

Contents:

Campylobacter jejuni Isolation/Enumeration from Environmental Samples

Recovery of Campylobacter spp. from Food and Environmental Sources

Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni

Drug Resistance Detection in Campylobacter

Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni

Identification of Ligand-receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies

Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni

Method for the Successful Crystallization of the Ferric Uptake Regulator from Campylobacter jejuni

Methods for Initial Characterization of Campylobacter jejuni Bacteriophages

Methods to Assess the Direct Interaction of C. jejuni with Mucins

Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells

Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface

Characterisation of Ligand-receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies

Using Galleria Mellonella as an Infection Model for Campylobacter jejuni Pathogenesis

Mouse Models for Campylobacter jejuni Colonization and Infection

Metabolomic Analysis of Campylobacter jejuni by Direct Injection Electrospray Ionisation Mass Spectrometry

Methods for Genome-wide Methylome Profiling of Campylobacter jejuni

Characterizing Glycoproteins by Mass Spectrometry in Campylobacter jejuni

Methods for Proteome Analysis of Campylobacter jejuni using 2-D Electrophoresis

Analyzing Prokaryotic RNA-seq Data: A Case Study Identifying Holo-Fur Regulated Genes in Campylobacter jejuni

Generation and Screening of an Insertion Sequencing-compatible Mutant Library of Campylobacter jejuni. .

Other Format:

Printed edition:

ISBN:

9781493965366

Access Restriction:

Restricted for use by site license.