High-Resolution Imaging of Cellular Proteins : Methods and Protocols / edited by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski.

Format:

Book

Contributor:

Schwartzbach, Steven D., editor.

Skalli, Omar, editor.

Schikorski, Thomas, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1474.

Methods in Molecular Biology, 1064-3745 ; 1474

Language:

English

Subjects (All):

Medicine.

Radiology.

Physical Description:

1 online resource (XIII, 366 pages) : 45 illustrations, 21 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.

Contents:

Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture

Antibody Production with Synthetic Peptides

Production and Purification of Polyclonal Antibodies

Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2 and Streptavidin

Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications

Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy

Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells

Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses

Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells

Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging

Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins

Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas

Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells

Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues

Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae

Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes

Pre-Embedding Nanogold Silver and Gold Intensification

Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval

Pre- and Post-Embedding Immunogold Labeling of Tissue Sections

Immuno-Gold Labelling for Scanning Electron Microscopy

Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope

Functional Nanoscale Imaging Of Synaptic Vesicle Cycling With Superfast Fixation.

Other Format:

Printed edition:

ISBN:

9781493963522

Access Restriction:

Restricted for use by site license.