SUMO : Methods and Protocols / edited by Manuel S. Rodriguez.

Format:

Book

Contributor:

Rodriguez, Manuel S., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1475.

Methods in Molecular Biology, 1064-3745 ; 1475

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Physical Description:

1 online resource (XI, 306 pages) : 54 illustrations, 34 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls. Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system.

Contents:

Concepts and Methodologies to Study Protein SUMOylation: An Overview

The Regulation of Chromatin by Dynamic SUMO Modifications

Reconstitution of the Recombinant RanBP1 SUMO E3 Ligase Complex

Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria

A Fluorescent In Vitro Assay to Investigate Paralog Specific SUMO Conjugation

Identification and Characterization of SUMO-SIM Interactions

Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions between SUMO-Traps and Mono or PolySUMO Moieties

Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins

In Vitro Characterization of Chain Depolymerization Activities of SUMO-Specific Proteases

Detection of Protein SUMOylation In Situ by Proximity Ligation Assays

In Situ SUMOylation and DeSUMOylation Assays: Fluorescent Methods to Visualize SUMOylation and DeSUMOylation in Permeabilized Cells

Analysis of SUMOylated Proteins in Cells and In Vivo Using BioSUMO Strategy

Label-Free Identification and Quantification of SUMO Target Proteins

The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding Proteins

Isolation of In Vivo SUMOylated Chromatin-Bound Proteins

Identification of Substrates of Protein-Group SUMOylation

Tools to Study SUMO Conjugation in Caenorhabditis elegans

Purification of SUMO Conjugates from Arabidopsis for Mass Spectrometry Analysis

Detection of SUMOylation in Plasmodium falciparum

Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1.

Other Format:

Printed edition:

ISBN:

9781493963584

Access Restriction:

Restricted for use by site license.