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Liposomes : Methods and Protocols / edited by Gerard G.M. D'Souza.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
D'Souza, Gerard G. M., editor.
SpringerLink (Online service)
Series:
Methods in molecular biology 1064-3745 ; 1522.
Methods in Molecular Biology, 1064-3745 ; 1522
Language:
English
Subjects (All):
Medicine.
Pharmacology.
Physical Description:
1 online resource (XI, 285 pages) : 59 illustrations, 13 illustrations in color.
Edition:
Second edition 2017.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2017.
System Details:
text file PDF
Summary:
This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry. .
Contents:
Liposomes Came First: The Early History of Liposomology
Thin Film Hydration followed by Extrusion Method for Liposome Preparation
Preparation of DRV Liposomes
Method of Simultaneous Analysis of Liposome Components by HPTLC/FID
Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Liposome Formulations of Hydrophobic Drugs
A "Dock and Lock" Approach to Preparation of Targeted Liposomes
Coupling of Ligands to the Liposome Surface by Click Chemistry
Elastic Liposomes for Topical and Transdermal Drug Delivery
Determination of the Sub-Cellular Distribution of Liposomes using Confocal Microscopy
Environmental Scanning Electron Microscope Imaging of Vesicle Systems
Liposome Biodistribution via Europium Complexes
Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide Therapy
Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes
Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes
Long-Circulating, pH-Sensitive Liposomes
Anionic pH-Sensitive Lipoplexes
Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Preparation and Physical Characterization of DNA Binding Cationic Liposomes
Fluorescence Resonance Energy Transfer (FRET) Based Analysis of Lipoplexes
Targeted Magnetic Liposomes Loaded with Doxorubicin
Stable Discoidal Bicelles - A Platform of Lipid Nanocarriers for Cellular Delivery.
Other Format:
Printed edition:
ISBN:
9781493965915
Access Restriction:
Restricted for use by site license.

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