ERK Signaling : Methods and Protocols / edited by Gerardo Jimenez.

Format:

Book

Contributor:

Jimenez, Gerardo (Research professor), editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1487.

Methods in Molecular Biology, 1064-3745 ; 1487

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Physical Description:

1 online resource (XII, 440 pages) : 40 illustrations, 21 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities; proteomic and genome-wide analyses of ERK signaling targets; cell biological, genetic, quantitative and imaging approaches in cells and model organisms; and integrated mathematical modeling of the ERK pathway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ERK Signaling: Methods and Protocols will aid and stimulate further advances in the vibrant field of ERK signaling. < .

Contents:

How Genetics has Helped Piece Together the MAPK Signaling Pathway

In Vitro Enzyme Kinetics Analysis of EGFR

High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells

Structural Studies of ERK2 Protein Complexes

Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk

Assaying Activation and Subcellular Localization of ERK in Cells and Tissues

Detection and Functional Analysis of SUMO-Modified MEK

Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag

High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates

Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE

Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation

Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions

The Nuclear Translocation of ERK

Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)

Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors

Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers

Co-Culture Activation of MAP Kinase in Drosophila S2 Cells

Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling

3D Organotypic Culture Model to Study Components of ERK Signaling

Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway

Genome-Wide Analysis of RAS/ERK Signaling Targets

Probing Chromatin Modifications in Response to ERK Signaling

Analyzing pERK Activation during Planarian Regeneration

Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development

Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images

Using CRISPR-Cas9 to Study ERK Signaling in Drosophila

Analyzing ERK Signal Dynamics during Zebrafish Somitogenesis

Modeling RASopathies with Genetically Modified Mouse Models

Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.

Other Format:

Printed edition:

ISBN:

9781493964246

Access Restriction:

Restricted for use by site license.