Advances and Clinical Practice in Pyrosequencing / edited by Guohua Zhou, Qinxin Song.

Format:

Book

Contributor:

Zhou, Guohua, editor.

Song, Qinxin, editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2448

Springer Protocols Handbooks, 1949-2448

Language:

English

Subjects (All):

Medicine.

Human genetics.

Physical Description:

1 online resource (XIV, 407 pages) : 156 illustrations, 44 illustrations in color.

Edition:

First edition 2016.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology. Divided into five parts, the book's thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturization of pyrosequencing equipment, as well as various applications. As part of the Springer Protocols program, chapters contain the kind of detail and practical implementation advice to guarantee successful results in the lab. Comprehensive and thorough, Advances and Clinical Practice in Pyrosequencing serves as a valuable reference for researchers who are engaged in personalized medicine, disease control, and DNA diagnosis in numerous other fields.

Contents:

Preparation of single-stranded DNA for pyrosequencing by LATE-PCR

A simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly

Improvement of LATE-PCR to prepare pyrosequencing template

Pyrosequencing-templates generated by nicking PCR products with nicking endonuclease

Pyrosequencing-templates generated by asymmetric Nucleic Acid Sequence-Based Amplification (asymmetric-NASBA)

Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing

Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing

A novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit

Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample

A gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactions directly from double-stranded PCR products

Improvement of pyrosequencing to allow multiplex SNP typing in a program

Improvement of pyrosequencing-sensitivity based on the capture of free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase

Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing

Preparation of thermalstable biotinlyted firefly luciferase and its application in pyrosequencing

Expression of thermostable recombinant luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system

Expression and purification of ATP sulfurylase from saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing

Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing

Expression of PPDK from Microbispora Rosea Subsp.aerata in E.coli and its application in pyrosequencing

Construction of 3-plex barcodes for differential gene-expression analysis with pyrosequencing

Development of pyrosequencing-based multiplex bioassay by designing barcodes encoded with artificially designed sequences

Quantitatively discriminating multiplexed LAMP products with pyrosequencing-based bio-barcodes

Multiplex ligation-dependent sequence-tag amplification coupled with pyrosequencing for CNV analysis

Pyrosequencer miniaturized with capillaries to deliver deoxynucleotides

Pyrosequencer with a senor of a photo-diode array

Pyrosequencing on acryl-modified glass chip

Pyrosequencing on-chip based on a gel-based solid-phase amplification

Prenatal diagnosis of trisomy 21 by quantitatively pyrosequencing heterozygotes using amniotic fluid as starting material of PCR

Comparative gene expression analysis of breast-cancer related genes by multiplex pyrosequencing coupled with sequence-barcodes

MicroRNA quantification by pyrosequencing with a sequence-tagged stem-loop RT primer.- Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer

Genotyping of pathogenic serotypes of S. suis with pyrosequencing

Differential gene expression analysis of breast cancer by combining sequence-tagged reverse-transcription PCR with pyrosequencing

Detection of avian influenza A virus by pyrosequencing

Genotyping of the alcohol dehydrogenase gene by pyrosequencing coupled with improved LATE-PCR using human whole blood as starting material

Pyrosequencing on a single cell.

Other Format:

Printed edition:

ISBN:

9781493933082

Access Restriction:

Restricted for use by site license.