Animal Coronaviruses / edited by Leyi Wang.

Format:

Book

Contributor:

Wang, Leyi, editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2448

Springer Protocols Handbooks, 1949-2448

Language:

English

Subjects (All):

Medicine.

Infectious diseases.

Physical Description:

1 online resource (X, 218 pages) : 25 illustrations, 15 illustrations in color.

Edition:

First edition 2016.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

Contents:

Animal Coronaviruses: A Brief Introduction

Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues

Virus Neutralization Assay for Turkey Coronavirus Infection

Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli

Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen

Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus

Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses

Real-Time RT-PCR Detection of Equine Coronavirus

Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction

Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus

An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes

A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus

Real-Time Reverse Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues

PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene

Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis

Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction

Molecular Characterization of Canine Coronavirus

Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform

Next Generation Sequencing for Porcine Coronaviruses.

Other Format:

Printed edition:

ISBN:

9781493934140

Access Restriction:

Restricted for use by site license.