The Mitotic Spindle : Methods and Protocols / edited by Paul Chang, Ryoma Ohi.

Format:

Book

Contributor:

Chang, Paul, editor.

Ohi, Ryoma, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1413.

Methods in Molecular Biology, 1064-3745 ; 1413

Language:

English

Subjects (All):

Life sciences.

Cell membranes.

Microscopy.

Physical Description:

1 online resource (XIII, 424 pages) : 79 illustrations, 52 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field. .

Contents:

Using Fluorescence Microscopy to Study Mitosis

Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation

Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts

Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro

Imaging and Quantifying the Dynamics of γ-Tubulin at Microtubule Minus Ends in Mitotic Spindles

Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy

Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size

In Vitro Kinetochore Assembly

Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes

Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells

Studying Kinetochores In Vivo Using FLIM-FRET

Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies

A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion

Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation

Analyzing Spindle Positioning Dynamics in Cultured Cells

Quantification of Mitotic Chromosome Alignment

Imaging Mitosis in the Moss Physcomitrella Patens

Small Molecule Approach to Study the Function of Mitotic Kinesins

Identification and Characterization of Mitotic Spindle-Localized Transcripts

Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization

Studying Kinetochore Kinases

Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics

Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer

Generation and Purification of Tetraploid Cells

Anti-Microtubule Drugs.

Other Format:

Printed edition:

ISBN:

9781493935420

Access Restriction:

Restricted for use by site license.