RNA-Protein Complexes and Interactions : Methods and Protocols / edited by Ren-Jang Lin.

Format:

Book

Contributor:

Lin, Ren-Jang, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1421.

Methods in Molecular Biology, 1064-3745 ; 1421

Language:

English

Subjects (All):

Life sciences.

Proteins.

Physical Description:

1 online resource (XIII, 288 pages) : 49 illustrations, 24 illustrations in color.

Edition:

First edition 2016.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand. After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins, biochemical methods for measuring direct RNA-protein contact, as well as various new or innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, RNA-Protein Complexes and Interactions: Methods and Protocols provides a set of useful protocols, both basic and advanced, designed to inspire researchers working with RNA and RNA-interacting proteins.

Contents:

Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2

Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)

Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro

Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting

Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture

Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts

In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly

Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases

Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles

Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture

Measuring mRNA Translation by Polysome Profiling

Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq

Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment

Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks

Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking

Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX

mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins

Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors

Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5' Splice-Site Base-Pairing

Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter.

Other Format:

Printed edition:

ISBN:

9781493935918

Access Restriction:

Restricted for use by site license.