Chromosome Architecture : Methods and Protocols / edited by Mark C. Leake.

Format:

Book

Contributor:

Leake, Mark C., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1431.

Methods in Molecular Biology, 1064-3745 ; 1431

Language:

English

Subjects (All):

Medicine.

Human genetics.

Physical Description:

1 online resource (X, 290 pages) : 77 illustrations, 65 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

New Advances in Chromosome Architecture

Single Molecule Narrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose Signal Transduction Of Live Yeast Cells

Single-Molecule Imaging to Characterise the Transport Mechanism of the Nuclear Pore Complex

Using Fluorescence Recovery after Photobleaching (FRAP) To Study Dynamics Of The Structural Maintenance Of Chromosome (SMC) Complex In Vivo

Imaging DNA Structure by Atomic Force Microscopy

Investigating Bacterial Chromosome Architecture Using Epifluorescence Microscopy

Transverse Magnetic Tweezers Allowing Coincident Epi-Fluorescence Microscopy On Horizontally Extended DNA

Probing Chromosome Dynamics in Bacillus Subtilis

Systems Biology Approaches for Understanding Genome Architecture

In Vivo And In Situ Replication Labeling Methods For Super-Resolution Structured Illumination Microscopy Of Chromosome Territories And Chromatin Domains

DNA-Protein Interactions Studied Directly Using Single Molecule Fluorescence Imaging Of Quantum Dot Tagged Proteins Moving On DNA Tightropes

Escherichia Coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis

Bacterial Chromosome Dynamics by Locus Tracking In Fluorescence Microscopy

Biophysical Characterization of Chromatin Remodelling Protein CHD4

Atomistic Molecular Dynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup, Performance and Analysis

Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells

Studying the Dynamics of Chromatin Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy

Intra Nuclear Single Particle Tracking (I-SPT) To Reveal the Functional Architecture Of Chromosomes

Visualizing Bacillus Subtilis during Vegetative Growth and Spore Formation. .

Other Format:

Printed edition:

ISBN:

9781493936311

Access Restriction:

Restricted for use by site license.