Proteostasis : Methods and Protocols / edited by Rune Matthiesen.

Format:

Book

Contributor:

Matthiesen, Rune, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1449.

Methods in Molecular Biology, 1064-3745 ; 1449

Language:

English

Subjects (All):

Life sciences.

Proteins.

Physical Description:

1 online resource (XVI, 488 pages) : 94 illustrations, 60 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteostasis: Methods and Protocols aims to become a reference book on proteostasis in human health.

Contents:

UPS Activation in the Battle against Aging and Aggregation-Related Diseases: An Extended Review

Review And Literature Mining On Proteostasis Factors' And Cancer

Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role Of Usp45 in the Retina

Immunodepletion and Immunopurification as Approaches for CSN Research

Studying Protein Ubiquitylation in Yeast

Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein

In Vitro Ubiquitination - Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays

Isolation of the Ubiquitin-Proteome From Tumour Cell Lines and Primary Cells Using Tubes

Tubes-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome

Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples

Method for the Purification of Endogenous Unanchored Polyubiquitin Chains

Fluorescent Tools for In Vivo Studies on the Ubiquitin-Proteasome System

Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells

Monitoring Ubiquitin-Coated Bacteria Via Confocal Microscopy

Detection and Analysis of Cell Cycle-Associated Apc/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo

Detection and Analysis Of Sumoylation Substrates In Vitro and In Vivo. - Detection of Protein-Protein Interactions and Post-Translational Modifications using the Proximity Ligation Assay: Application to the Study of the Sumo Pathway

Dissecting of Sumo Dynamics by Mass Spectrometry

Isolation of Lysosomes from Mammalian Tissues and Cultured Cells

Analysis Of Relevant Parameters For Autophagic Flux Using Hela Cells Expressing Egfp-Lc3

Analysis of Protein Oligomeric Species by Sucrose Gradients

Analysis of Protein Oligomerization by Electrophoresis.-Blot-Ms of Carbonylated Proteins: A Tool to Identify Oxidized Proteins

Quantitation of Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry

A Simple Protocol for High Efficiency Protein Isolation after RNA Isolation from Mouse Thyroid and Other Very Small Tissue Samples

Monitoring Target Engagement Of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future

Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues

High-Throughput Sirna Screening Applied to the Ubiquitin-Proteasome System

High-Throughput Yeast-Based Reporter Assay to Identify Compounds with Anti-Inflammatory Potential

Using Alphascreen ® to Identify Small Molecule Inhibitors Targeting a Conserved Host-Pathogen Interaction

Global MS-Based Proteomics Drug Profiling.

Other Format:

Printed edition:

ISBN:

9781493937561

Access Restriction:

Restricted for use by site license.