The Tumor Microenvironment : Methods and Protocols / edited by Josie Ursini-Siegel, Nicole Beauchemin.

Format:

Book

Contributor:

Ursini-Siegel, Josie, editor.

Beauchemin, Nicole, 1947- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1458.

Methods in Molecular Biology, 1064-3745 ; 1458

Language:

English

Subjects (All):

Medicine.

Cancer--Research.

Cancer.

Physical Description:

1 online resource (XII, 356 pages) : 38 illustrations, 19 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various "omics"-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology. Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.

Contents:

Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible

Laser Capture Microdissection as a Tool to Study Tumor Stroma

Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging

Studies on the Tumor Vasculature and Coagulant Microenvironment

A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment

Measuring Vascular Permeability In Vivo

Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL)

Analyzing the Tumor Microenvironment by Flow Cytometry

Detecting Secreted Analytes from Immune Cells: An Overview of Technologies

Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-Parameter Flow Cytometry-Based Sorting

Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes

Methods to Evaluate the Anti-Tumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies

Isolation and Characterization of Low Vs. High-Density Neutrophils in Cancer

Analysis of Extracellular Vesicles in the Tumor Microenvironment

Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disc Confocal Microscopy

Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice

Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer

Modeling Breast Tumor Development with a Humanized Mouse Model

CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice

Metabolomics Analyses of Cancer Cells in Controlled Microenvironments

Analysis of the Tumor Microenvironment Transcriptome Via NanoString mRNA and miRNA Expression Profiling

RNA-Seq as a Tool to Study the Tumor Microenvironment

Sample Preparation for Mass Spectrometry Analysis of Protein-Protein Interactions in Cancer Cell Lines and Tissues.

Other Format:

Printed edition:

ISBN:

9781493938018

Access Restriction:

Restricted for use by site license.