Inflammation : Methods and Protocols / edited by Björn E. Clausen, Jon D. Laman.

Format:

Book

Contributor:

Clausen, Björn E., editor.

Laman, Jon D., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1559.

Methods in Molecular Biology, 1064-3745 ; 1559

Language:

English

Subjects (All):

Medicine.

Immunology.

Physical Description:

1 online resource (XVI, 464 pages) : 110 illustrations, 82 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated inflammatory disease models affecting barrier organs to the environment; the skin, the lung, and the intestinal and oral mucosa. The fourth section illustrates inflammatory disease models of the brain, joints, and vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inflammation: Methods and Protocols aims to inspire the experienced investigator and the young experimenter alike to disentangle the fascinating process of inflammation.

Contents:

Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models

Hapten-specific T cell-mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate

Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry

Visualization of the T Cell Response in Contact Hypersensitivity

Ultraviolet Radiation-induced Immunosuppression - Induction of Regulatory T cells

Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model

Xenotransplantation Model of Psoriasis

A Mouse Model for Atopic Dermatitis using Topical Application of Vitamin D3 or of its Analog MC9039 Particle Bombardment of Ex-vivo Skin to Deliver DNA and Express Proteins

Mouse Models of Asthma

Subcutaneous and Sublingual Immunotherapy in a Mouse Model of Allergic Asthma

Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse

Induction and Analysis of Bronchus-associated Lymphoid Tissue

Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodes

Isolation and Identification of Intestinal Myeloid Cells

Monitoring and Modulation of Inducible Foxp3+ Regulatory T-cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon

Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria

Analysis of Leukocytes in Oral Mucosal Tissues

Optimized Mouse Models for Liver Fibrosis

Monitoring of Chemically Induced Colitis

Oxazolone-induced Intestinal Inflammation in Adult Zebrafish

High Dimensional Cytometry of Central Nervous System Leukocytes During Inflammation

Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System

Investigating the Lymphatic Drainage of the Brain: Essential Skills and Tools

Quantitative Assessment of Cerebral Basement Membranes using Electron Microscopy

Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish

Experimental Arthritis Mouse Models Driven by Adaptive and/or Innate Inflammation

Pain Relief in Nonhuman Primate Models of Arthritis

Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis

Assessment of Vascular Dysfunction and Inflammation Induced by Angiotensin II in Mice.

Other Format:

Printed edition:

ISBN:

9781493967865

Access Restriction:

Restricted for use by site license.