Histochemistry of Single Molecules : Methods and Protocols / edited by Carlo Pellicciari, Marco Biggiogera.

Format:

Book

Contributor:

Pellicciari, Carlo, editor.

Biggiogera, Marco, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1560.

Methods in Molecular Biology, 1064-3745 ; 1560

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Physical Description:

1 online resource (XIV, 341 pages) : 70 illustrations, 59 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets. It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.

Contents:

Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes

Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues

Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

Histochemical Demonstration of Tripeptidyl Aminopeptidase I

Enzyme Histochemistry for Functional Histology in Invertebrates

Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future

Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads

Lectin Histochemistry for Metastasizing and Non-Metastasizing Cancer Cells

The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications

Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain

Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration

Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue

Tissue Fixation and Processing for the Histological Identification of Lipids

Staining Methods for Normal and Regenerative Myelin in the Nervous System

Nile Red Staining of Neutral Lipids in Yeast

Staining of Lipid Droplets with Monodansylpentane

Fluorochromes for DNA Staining and Quantitation

Osmium Ammine for Staining DNA at Electron Microscopy

DNA Labeling at Electron Microscopy

Visualizing RNA at Electron Microscopy by Terbium Citrate

Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks

Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins

Histochemical Analysis of Plant Secretory Structures

A Histochemical Technique for the Detection of Annonaceous Acetogenins.

Other Format:

Printed edition:

ISBN:

9781493967889

Access Restriction:

Restricted for use by site license.