Morpholino Oligomers : Methods and Protocols / edited by Hong M. Moulton, Jon D. Moulton.

Format:

Book

Contributor:

Moulton, Hong M., editor.

Moulton, Jon D. (Jon Daniel), 1962- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1565.

Methods in Molecular Biology, 1064-3745 ; 1565

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Physical Description:

1 online resource (X, 284 pages) : 60 illustrations, 38 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.

Contents:

Invention and Early History of Morpholinos: From Pipe Dream to Practical Products

Making a Morpholino Experiment Work: Controls, Favoring Specificity, Improving Efficacy, Storage, and Dose

Aggregation and Disaggregation of Morpholino Oligomers in Solution

End-Modifications on Morpholino Oligos

Inducible Inhibition of Gene Function with Photomorpholinos

Blocking Zebrafish microRNAs with Morpholinos

Using Morpholinos to Examine Gene Function During Fin Regeneration

Using Morpholinos to Probe Gene Networks in Sea Urchin

Manipulating Gene Expression in the Chick Embryo

Inhibition of Bacterial Growth by Peptide-conjugated Morpholino Oligomers

Use of Translation Blocking Morpholinos for Gene Knock-Down in Giardia lamblia

Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos

Targeting Functional Non-coding RNAs

Use of Morpholino Oligomers for Pretargeting

Diagnostic Applications of Morpholinos and Label-free Electrochemical Detection of Nucleic Acids

Intranasal Delivery of Peptide-Morpholinos to Knockdown Influenza Host Factors in Mice

Systemic Delivery of Morpholinos to Skip Multiple Exons in a Dog Model of Duchenne Muscular Dystrophy

In vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina.<intracerebroventricular delivery="" in="" mice="" for="" motor="" neuron="" diseases.-="" of="" morpholino="" antisense="" oligonucleotides="" to="" a="" developing="" ovine="" conceptus="" via="" luminal="" injection="" into="" ligated="" uterine="" horn.-="" surface="" plasmon="" resonance-based="" concentration="" determination="" assay:="" label-free="" and="" antibody-free="" quantification="" morpholinos.-="" ultrasensitive="" hybridization-based="" elisa="" method="" the="" phosphorodiamidate="" biological="" samples. .

Other Format:

Printed edition:

ISBN:

9781493968176

Access Restriction:

Restricted for use by site license.