Thermogenic Fat : Methods and Protocols / edited by Jun Wu.

Format:

Book

Contributor:

Wu, Jun (Biologist), editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1566.

Methods in Molecular Biology, 1064-3745 ; 1566

Language:

English

Subjects (All):

Life sciences.

Cytology.

Physical Description:

1 online resource (XI, 214 pages) : 42 illustrations, 30 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.

Contents:

Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue

Isolation of Mouse Stromal Vascular Cells for Monolayer Culture

Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells

Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes

Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells

Isolation of Immune Cells from Adipose Tissue

Differentiation and Metabolic Interrogation of Human Adipocytes

Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue

Genetic Mouse Models: The Powerful Tools to Study Fat Tissues

Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function

Bioenergetic Analyses in Adipose Tissue

Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry

Isolation and Patch-Clamp of Primary Adipocytes

In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver

Identification and Quantification of Human Brown Adipose Tissue

Designing 3D Adipospheres for Quantitative Metabolic Study

Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems

Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.

Other Format:

Printed edition:

ISBN:

9781493968206

Access Restriction:

Restricted for use by site license.