Mitochondria : Practical Protocols / edited by Dejana Mokranjac, Fabiana Perocchi.

Format:

Book

Contributor:

Mokranjac, Dejana, editor.

Perocchi, Fabiana, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1567.

Methods in Molecular Biology, 1064-3745 ; 1567

Language:

English

Subjects (All):

Life sciences.

Cytology.

Physical Description:

1 online resource (XI, 409 pages) : 77 illustrations, 46 illustrations in color.

Edition:

Second edition 2017.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondria: Practical Protocols, Second Edition aims to be useful for beginners as well as for experienced researchers in the field.

Contents:

A Guide to Computational Methods for Predicting Mitochondrial Localization

Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues

Isolation of Mitochondria From Saccharomyces Cerevisiae

Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes

Isolation Of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue

Label-Free Quantitative Analysis of Mitochondrial Proteomes using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and 'Total Protein Approach'

Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry

Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells

Chemical Crosslinking in Intact Mitochondria

Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression

Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast

Localizing Mrnas Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation

Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues using Seahorse XF96 Analyzer

Application of FRET-Based Biosensor "Ateam" for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells

A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake

New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis Elegans

Single Molecule Tracking and Localization Of Mitochondrial Protein Complexes in Live Cells

Analysis of Yeast Mitochondria by Electron Microscopy

Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-Tomography

Assays for Mitophagy in Yeast

Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis Elegans

Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells

Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by Qrt-PCR and Rnaseq

Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines. .

Other Format:

Printed edition:

ISBN:

9781493968244

Access Restriction:

Restricted for use by site license.