Protein Terminal Profiling : Methods and Protocols / edited by Oliver Schilling.

Format:

Book

Contributor:

Schilling, Oliver, 1975- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1574.

Methods in Molecular Biology, 1064-3745 ; 1574

Language:

English

Subjects (All):

Life sciences.

Proteins.

Physical Description:

1 online resource (XII, 268 pages) : 53 illustrations, 37 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. < Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.

Contents:

[14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay

DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity

SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation

Profiling of Protein N-Termini and their Modifications in Complex Samples

Protease Substrate Profiling by N-Terminal COFRADIC

Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification

Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types

System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates

Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC

ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling

Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP)

Multiplexed Protease Specificity Profiling Using Isobaric Labeling

FPPS - Fast Profiling of Protease Specificity

Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics

Prediction of Proteases Involved in Peptide Generation

Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches

Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors

Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method

Synthesis and Application of Activity-Based Probes for Proteases.

Other Format:

Printed edition:

ISBN:

9781493968503

Access Restriction:

Restricted for use by site license.