Lipidomics : Methods and Protocols / edited by Sanjoy K. Bhattacharya.

Format:

Book

Contributor:

Bhattacharya, Sanjoy K. (Professor of ophthalmology), editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1609.

Methods in Molecular Biology, 1064-3745 ; 1609

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Physical Description:

1 online resource (XII, 281 pages) : 74 illustrations, 41 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume explores lipidomics through protocols that focus on areas of utility, techniques, and bioinformatics advancements. The protocols in this book cover topics such as isolation of specific membranes and specialized fractionation of subcellular compartments, and computational and functional analysis of lipid metabolizing enzymes. Each protocol also discusses methods of dealing with fractionated organelles-cells to whole organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Lipidomics: Methods and Protocols is a useful and valuable resource for biochemists, molecular biologists, and neuroscientists interested in neurology, ophthalmology and vision sciences, as well as mass spectrometrists with interest in disease discovery. .

Contents:

Lipid Sample Preparation for Biomedical Research

Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays

Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells

Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation

Sample Preparation and Analysis for Imaging Mass Spectrometry

Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay Based Method

High-Performance Chromatographic Separation of Cerebrosides

Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography

Utility of Moderate and High Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification

A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry

Combined Use of MALDI-TOF Mass Spectrometry and 31PNMR Spectrometry for Analysis of Phospholipids

Global Monitoring of Mammalian Lipidome by Quantitative Shotgun Lipodomics

Bioinformatics Pertinent to Lipid Analysis in Biological Samples

LC-MS-Based Lipidomics and Automated Identification of Lipids using the LipidBlast In-Silico MS/MS Library

Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa

Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains

Computational Functional Analysis of Lipid Metabolic Enzymes

Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells

Purification and Validation of Lipid Transfer Proteins

Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells

Sonication-Based Basic Protocol for Liposome Synthesis

On Electrochemical Methods for Determination of Protein-Lipid Interaction

Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate. .

Other Format:

Printed edition:

ISBN:

9781493969968

Access Restriction:

Restricted for use by site license.