Cholesterol Homeostasis : Methods and Protocols / edited by Ingrid C. Gelissen, Andrew J. Brown.

Format:

Book

Contributor:

Gelissen, Ingrid C., editor.

Brown, A. J. (Andrew J.), editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1583.

Methods in Molecular Biology, 1064-3745 ; 1583

Language:

English

Subjects (All):

Life sciences.

Cytology.

Physical Description:

1 online resource (XI, 300 pages) : 64 illustrations, 37 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell. Chapters also cover techniques for studying the regulation of cholesterol homeostasis at both the transcriptional and post-translational levels, as well as studying the membrane topology and structure of cholesterol-related proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cholesterol Homeostasis: Methods and Protocol aims to provide key techniques in tackling the investigation of cholesterol homeostasis.

Contents:

An Overview of Cholesterol Homeostasis

Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol Binding Domains in Membrane Proteins

Structural Stringency of Cholesterol for Membrane Protein Function utilizing Stereoisomers as Novel Tools: A Review

Manipulating Cholesterol Status within Cells

Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells

The use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolaemia and Atherosclerosis

CRISPR/Cas9-mediated Generation of Niemann-Pick C1 Knockout Cell Line

Quantitative Measurement of Cholesterol in Cell Population Using Flow Cytometry and Fluorescent Perfringolysin O*

Transport Assays for Sterol-binding Proteins: Stopped-flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein

Synthesis and Live-cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport

Measurement of Cholesterol Transfer from Lysosome to Peroxisomes Using an In Vitro Reconstitution Assay

Measurement of Mitochondrial Cholesterol Import using a Mitochondria-targeted CYP11A1 Fusion Construct

Identifying Sterol Response Elements within Promoters of Genes

Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion

Determining the Topology of Membrane-bound Proteins using PEGylation

Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry

Sterol Analysis by Quantitative Mass Spectrometry

Measurement of Rates of Cholesterol and Fatty Acid Synthesis in vivo using Tritiated Water

Methods for Monitoring ABCA1-dependent Sterol Release

ABC-transporter Mediated Sterol Export from Cells using Radio-Labeled Sterols

Measurement of Macrophage-specific In Vivo Reverse Cholesterol Transport in Mice.

Other Format:

Printed edition:

ISBN:

9781493968756

Access Restriction:

Restricted for use by site license.