Lipidomics / edited by Paul Wood.

Format:

Book

Contributor:

Wood, Paul, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 125.

Neuromethods, 0893-2336 ; 125

Language:

English

Subjects (All):

Medicine.

Neurosciences.

Physical Description:

1 online resource (XII, 248 pages) : 95 illustrations, 61 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells (lymphocytes and oocytes). Lipidomics guides readers through chapters on direct-flow and chromatographic methods (SFC, UHPLC, HPTLC, ion-mobility); derivatization methods for lipids (amines, fatty aldehydes and ketones); TOF-SIMS imaging of lipids; and characterization of lipid transfer proteins. Additional chapters also provide an authoritative overview of lipidomics strategies and a detailed review of high-resolution mass spectrometric methods are included in this volume. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Lipidomics aims to ensure successful results in the further study of this vital field.

Contents:

Introduction and Overview of Lipidomic Strategies

Non-targeted Lipidomics Utilizing Constant Infusion High Resolution ESI Mass Spectrometry

Ultrahigh-Resolution Lipid Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

MALDI-Imaging Mass Spectrometry of Brain Lipids

Ion-Mobility Mass Spectrometry for Lipidomics Applications

In vitro Assay to Extract Specific Lipid Types from Phospholipid Membranes using Lipid-Transfer Proteins: A Lesson from the Ceramide Transport Protein CERT

Quantification of Endogenous Endocannabinoids by LC-MS/MS

Lipid Profiling by Supercritical Fluid Chromatography/Mass Spectrometry

Mass Spectrometric Analysis of Lipid Hydroperoxides

Mass Spectrometric Determination of Fatty Aldehydes Exemplified by Monitoring the Oxidative Degradation of (2E)-hexadecenal in HepG2 Cell Lysates

CE Analysis of Phospholipid Headgroups

HPTLC-MALDI TOF MS Imaging Analysis of Phospholipids

Global UHPLC/HRMS Lipidomics Workflow for the Analysis of Lymphocyte Suspension Cultures

Ambient Lipidomic Analysis of Brain Tissue using Desorption Electrospray Ionization (DESI) Mass Spectrometry

Lipidomics Analyses of Oxygenated Metabolites of Polyunsaturated Fatty Acids

Derivatization of Fatty Aldehydes and Ketones: Girard's Reagent T (GRT)

Derivatization of Lipid Amines: Fluorenylmethyloxycarbonyl (FMOC)

High-Resolution Mass Spectrometry of Glycerophospholipid Oxidation Products. .

Other Format:

Printed edition:

ISBN:

9781493969463

Access Restriction:

Restricted for use by site license.