Pumps, channels, and transporters : methods of functional analysis / edited by Ronald J. Clarke, Mohammed A. A. Khalid.

Format:

Book

Contributor:

Clarke, Ronald J. (Ronald James), editor.

Khalid, Mohammed A. A. (A. Ali), editor.

Series:

Chemical analysis.

Chemical analysis : a series of monographs on analytical chemistry and its applications

Language:

English

Subjects (All):

Carrier proteins.

Ion pumps.

Ion channels.

Membrane proteins.

Biological transport.

Physical Description:

1 online resource (630 pages) : illustrations, tables.

Edition:

1st ed.

Place of Publication:

Hoboken, New Jersey : Wiley, 2015.

Summary:

Describes experimental methods for investigating the function of pumps, channels and transporters * Covers new emerging analytical methods used to study ion transport membrane proteins such as single-molecule spectroscopy * Details a wide range of electrophysiological techniques and spectroscopic methods used to analyze the function of ion channels, ion pumps and transporters * Covers state-of-the art analytical methods to study ion pumps, channels, and transporters, and where analytical chemistry can make further contributions

Contents:

Intro

TITLE PAGE

TABLE OF CONTENTS

PREFACE

LIST OF CONTRIBUTORS

1 INTRODUCTION

1.1 HISTORY

1.2 ENERGETICS OF TRANSPORT

1.3 MECHANISTIC CONSIDERATIONS

1.4 ION CHANNELS

1.5 ION PUMPS

1.6 TRANSPORTERS

1.7 DISEASES OF ION CHANNELS, PUMPS, AND TRANSPORTERS

1.8 CONCLUSION

ACKNOWLEDGMENTS

REFERENCES

2 STUDY OF ION PUMP ACTIVITY USING BLACK LIPID MEMBRANES

2.1 INTRODUCTION

2.2 FORMATION OF BLACK LIPID MEMBRANES

2.3 RECONSTITUTION IN BLACK LIPID MEMBRANES

2.4 THE PRINCIPLES OF CAPACITIVE COUPLING

2.5 THE GATED-CHANNEL CONCEPT

2.6 RELAXATION TECHNIQUES

2.7 ADMITTANCE MEASUREMENTS

2.8 THE INVESTIGATION OF CYTOPLASMIC AND EXTRACELLULAR ION ACCESS CHANNELS IN THE Na+,K+-ATPase

2.9 CONCLUSIONS

3 ANALYZING ION PERMEATION IN CHANNELS AND PUMPS USING PATCH-CLAMP RECORDING

3.1 INTRODUCTION

3.2 DESCRIPTION OF THE PATCH-CLAMP TECHNIQUE

3.3 PATCH-CLAMP MEASUREMENT AND ANALYSIS OF SINGLE CHANNEL CONDUCTANCE

3.4 DETERMINING ION SELECTIVITY AND RELATIVE PERMEATION IN WHOLE-CELL RECORDINGS

3.5 INFLUENCE OF VOLTAGE CORRECTIONS IN QUANTIFYING ION SELECTIVITY IN CHANNELS

3.6 ION PERMEATION PATHWAYS THROUGH CHANNELS AND PUMPS

3.7 CONCLUSIONS

4 PROBING CONFORMATIONAL TRANSITIONS OF MEMBRANE PROTEINS WITH VOLTAGE CLAMP FLUOROMETRY (VCF)

4.1 INTRODUCTION

4.2 DESCRIPTION OF THE VCF TECHNIQUE

4.3 PERSPECTIVES FROM EARLY MEASUREMENTS ON VOLTAGE-GATED K+ CHANNELS

4.4 VCF APPLIED TO P-TYPE ATPases

4.5 CONCLUSIONS AND PERSPECTIVES

5 PATCH CLAMP ANALYSIS OF TRANSPORTERS VIA PRE-STEADY-STATE KINETIC METHODS

5.1 INTRODUCTION

5.2 PATCH CLAMP ANALYSIS OF SECONDARY-ACTIVE TRANSPORTER FUNCTION

5.3 PERTURBATION METHODS.

5.4 EVALUATION AND INTERPRETATION OF PRE-STEADY-STATE KINETIC DATA

5.5 MECHANISTIC INSIGHT INTO TRANSPORTER FUNCTION

5.6 CASE STUDIES

5.7 CONCLUSIONS

6 RECORDING OF PUMP AND TRANSPORTER ACTIVITY USING SOLID-SUPPORTED MEMBRANES (SSM-BASED ELECTROPHYSIOLOGY)

6.1 INTRODUCTION

6.2 THE INSTRUMENT

6.3 MEASUREMENT PROCEDURES, DATA ANALYSIS, AND INTERPRETATION

6.4 P-TYPE ATPases INVESTIGATED BY SSM-BASED ELECTROPHYSIOLOGY

6.5 SECONDARY ACTIVE TRANSPORTERS

6.6 CONCLUSIONS

7 STOPPED-FLOW FLUORIMETRY USING VOLTAGE-SENSITIVE FLUORESCENT MEMBRANE PROBES

7.1 INTRODUCTION

7.2 BASICS OF THE STOPPED-FLOW TECHNIQUE

7.3 COVALENT VERSUS NONCOVALENT FLUORESCENCE LABELING

7.4 CLASSES OF VOLTAGE-SENSITIVE DYES

7.5 MEASUREMENT OF THE KINETICS OF THE Na+,K+-ATPase

7.6 CONCLUSIONS

8 NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

8.1 INTRODUCTION

8.2 COVALENTLY-INDUCED CHEMICAL SHIFT DIFFERENCES

8.3 SHIFT-REAGENT-INDUCED CHEMICAL SHIFT DIFFERENCES

8.4 pH-INDUCED CHEMICAL SHIFT DIFFERENCES

8.5 HYDROGEN-BOND-INDUCED CHEMICAL SHIFT DIFFERENCES

8.6 IONIC-ENVIRONMENT-INDUCED CHEMICAL SHIFT DIFFERENCES

8.7 RELAXATION TIME DIFFERENCES

8.8 DIFFUSION COEFFICIENT DIFFERENCES

8.9 SOME SUBTLE SPECTRAL EFFECTS

8.10 A CASE STUDY: THE STOICHIOMETRIC RELATIONSHIP BETWEEN THE NUMBER OF Na+ IONS TRANSPORTED PER MOLECULE OF GLUCOSE CONSUMED IN HUMAN RBCs

8.11 CONCLUSIONS

9 TIME-RESOLVED AND SURFACE-ENHANCED INFRARED SPECTROSCOPY

9.1 INTRODUCTION

9.2 BASICS OF IR SPECTROSCOPY

9.3 REFLECTION TECHNIQUES

9.4 APPLICATION TO ELECTRON-TRANSFERRING PROTEINS

9.5 TIME-RESOLVED IR SPECTROSCOPY

9.6 APPLICATIONS TO RETINAL PROTEINS

9.7 CONCLUSIONS

REFERENCES.

10 ANALYSIS OF MEMBRANE-PROTEIN COMPLEXES BY SINGLE-MOLECULE METHODS

10.1 INTRODUCTION

10.2 FLUOROPHORES FOR SINGLE PARTICLE LABELING

10.3 PRINCIPLES OF FLUORESCENCE CORRELATION SPECTROSCOPY

10.4 PRINCIPLE AND ANALYSIS OF SINGLE-MOLECULE IMAGING

10.5 COMPLEX DYNAMICS AND STOICHIOMETRY BY SINGLE-MOLECULE MICROSCOPY

10.6 FCS VERSUS SPT

11 PROBING CHANNEL, PUMP, AND TRANSPORTER FUNCTION USING SINGLE-MOLECULE FLUORESCENCE

11.1 INTRODUCTION

11.2 PRACTICAL CONSIDERATIONS

11.3 SMF IMAGING

11.4 SINGLE MOLECULE FÖRSTER RESONANCE ENERGY TRANSFER

11.5 SINGLE-MOLECULE COUNTING BY PHOTOBLEACHING

11.6 OPTICAL CHANNEL RECORDING

11.7 SIMULTANEOUS TECHNIQUES

11.8 SUMMARY

12 ELECTRON PARAMAGNETIC RESONANCE: SITE-DIRECTED SPIN LABELING

12.1 INTRODUCTION

12.2 BASICS OF THE EPR METHOD

12.3 STRUCTURAL AND DYNAMIC INFORMATION FROM SDSL-EPR

12.4 DISTANCE MEASUREMENTS

12.5 CHALLENGES

12.6 CONCLUSIONS

13 RADIOACTIVITY-BASED ANALYSIS OF ION TRANSPORT

13.1 INTRODUCTION

13.2 MEMBRANE PERMEABILITY FOR ELECTRONEUTRAL SUBSTANCES AND IONS

13.3 KINETIC CONSIDERATIONS

13.4 TECHNIQUES FOR ION FLUX MEASUREMENTS

13.5 KINETIC ANALYSIS OF ION TRANSPORTER PROPERTIES

13.6 SELECTED CATION TRANSPORTER STUDIES ON RED BLOOD CELLS

13.7 COMBINATION OF RADIOACTIVE ISOTOPE STUDIES WITH METHODS USING FLUORESCENT DYES

13.8 CONCLUSIONS

14 CATION UPTAKE STUDIES WITH ATOMIC ABSORPTION SPECTROPHOTOMETRY (AAS)

14.1 INTRODUCTION

14.2 OVERVIEW OF THE TECHNIQUE OF AAS

14.3 THE EXPRESSION SYSTEM OF XENOPUS LAEVIS OOCYTES FOR CATION FLUX STUDIES: PRACTICAL CONSIDERATIONS

14.4 EXPERIMENTAL OUTLINE OF THE AAS FLUX QUANTIFICATION TECHNIQUE.

14.5 REPRESENTATIVE RESULTS OBTAINED WITH THE AAS FLUX QUANTIFICATION TECHNIQUE

14.6 CONCLUDING REMARKS

15 LONG TIMESCALE MOLECULAR SIMULATIONS FOR UNDERSTANDING ION CHANNEL FUNCTION

15.1 INTRODUCTION

15.2 FUNDAMENTALS OF MD SIMULATION

15.3 SIMULATION DURATION AND SIMULATION SIZE

15.4 HISTORICAL DEVELOPMENT OF LONG MD SIMULATIONS

15.5 LIMITATIONS AND CHALLENGES FACING MD SIMULATIONS

15.6 EXAMPLE SIMULATIONS OF ION CHANNELS

15.7 CONCLUSIONS

INDEX

CHEMICAL ANALYSIS

END USER LICENSE AGREEMENT.

Notes:

"Ion-transporting membrane proteins permanently embedded in the membranes of cells or cell organelles can be grouped into three broad categories: channels, pumps, and transporters"--Chapter 1, introduction.

Includes bibliographical references at the end of each chapters and index.

Description based on print version record.

ISBN:

9781523110452

1523110457

9781119085133

1119085136

9781119085126

1119085128

9781119085140

1119085144

OCLC:

910009730